Ultrastructural Changes in Preimplantation Rabbit Embryos

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Ultrastructural changes in preimplantation rabbit embryos.

Follicular oocytes and penetrated eggs of several mammalian species have been the subject of numerous electron microscope studies (rat: Sotelo 1959, Sotelo and Porter 1959, Franchi 1960, Odor and Renninger 1960, Szollosi and Ris 1961, Guinea pig: Anderson and Beams 1960, Adams and Hertwig 1964, Austin 1965. Barron and Franklin 1968. cat: Liss 1964. man: Stegner and Waternberg 1961, Zamboni et a...

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Steroidogenesis in rabbit preimplantation embryos.

Rabbit preimplantation embryos were flushed from the reproductive tract at 24 hr (1- to 2-cell stage), 48 hr (morula), 72 hr (morula), 96 hr (blastocyst), 120 hr (blastocyst), and 144 hr (blastocyst) post coitum. At 168 hr (early postimplantation period), gestation sacs were excised, frozen, and sectioned in a cryostat. Delta5-3beta-Hydroxysteroid dehydrogenase [3(or 17)beta-hydroxysteroid:NAD(...

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Ultrastructural analysis of preimplantation lethal yellow (Ay/Ay) mouse embryos.

Embryos recovered at 62 and 80 h post coitum from reproductive tracts of yellow (Ay/a) and black (a/a) female mice mated to Ay/a males were examined ultrastructurally to define the developmental defect of lethal Ay homozygotes. No abnormalities were observed in five embryos from control matings (female a/a x male Ay/a). Of 24 morulae and blastocysts from Ay/a x Ay/a matings, six were observed t...

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Development of Rabbit Preimplantation Embryos under Thermal Stress in Vitro

The aim of our study was to examine the influence of short-term (6h) hyperthermia (41.5 °C and 42.5 °C) on the developmental capacity of preimplantation rabbit embryos in vitro. Rabbit embryos were isolated from superovulated and inseminated rabbit females after slaughter by flushing from oviducts of 19 hpc. The embryos were cultured in k-DMEM medium with the addition of 10 % fetal calf serum. ...

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Changes in responsiveness of preimplantation mouse embryos to serum.

Changes in uptake of radioactive uridine and its incorporation into RNA were determined in preimplantation mouse embryos, from the 2-cell to the blastocyst stage, as a measure of their responsiveness to extracellular conditions. Two media were tested, one contained serum and the other contained bovine serum albumen as a control. An increase in the acid-soluble pool occurred at the 8-cell stage ...

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ژورنال

عنوان ژورنال: CYTOLOGIA

سال: 1970

ISSN: 0011-4545,1348-7019

DOI: 10.1508/cytologia.35.319